Journal: iScience

Article Title: ATF6 ameliorates renal warm ischemia-reperfusion injury through FHL2-mediated NF-κB signaling pathway

doi: 10.1016/j.isci.2026.115173

Figure Lengend Snippet: ATF6 plays a protective role in renal ischemia-reperfusion injury (A–B) The levels of serum Cr and BUN in mice. (C–D) The levels of serum IL-6 and TNF-α in mice. (E–F) The representative images and kidney injury assessment score of HE staining of the kidney. Ceapin-A7 (10 μM) is an ATF6 inhibitor, and AA147 (10 μM) is an ATF6 agonist. Data represent mean ± SDs, and statistical analysis was performed using one-way ANOVA. (∗ p < 0.05).

Article Snippet: IL-6 ELISA kit , Boster , EK0412.

Techniques: Staining

Journal: iScience

Article Title: ATF6 ameliorates renal warm ischemia-reperfusion injury through FHL2-mediated NF-κB signaling pathway

doi: 10.1016/j.isci.2026.115173

Figure Lengend Snippet: ATF6 plays a protective role in renal ischemia-reperfusion injury (A–B) The levels of serum Cr and BUN in mice. (C–D) The levels of serum IL-6 and TNF-α in mice. (E–F) The representative images and kidney injury assessment score of HE staining of the kidney. (G–H) RT-qPCR analysis of TNF-α and IL-6 expression in cultured HK-2 in response to H/R treatment. (I–J) RT-qPCR analysis of IL-6 and TNF-α expression in cultured HK-2 in response to H/R using siATF6. Data represent mean ± SDs, and statistical analysis was performed using one-way ANOVA. (∗ p < 0.05).

Article Snippet: IL-6 ELISA kit , Boster , EK0412.

Techniques: Staining, Quantitative RT-PCR, Expressing, Cell Culture

Journal: Cell Death & Disease

Article Title: Chromatin accessibility analysis identifies the transcription factor ETV5 as a suppressor of adipose tissue macrophage activation in obesity

doi: 10.1038/s41419-021-04308-0

Figure Lengend Snippet: A BMDMs were transfected with siRNA negative control (siRNA NC) or siRNA specific to Etv5 (siRNA Etv5 ) 48 h and Il6 expression was assessed by RT-qPCR 48 h later. Il6 expression levels of Raw264.7 cells after knockdown of Etv5 using shRNA B or overexpression of Etv5 C . Il6 expression levels of Raw264.7 cells after knockdown of Etv5 using shRNA D or overexpression of Etv5 E under MMe induction condition. F Il6 expression levels of BMDMs after treatment with palmitate, glucose, and insulin alone or with a combination of these compounds for 24 h. G–I Measuring IL-6 protein levels in the supernatant of Raw264.7 cell culture by ELISA. Cells were treated with palmitate (P) or PGI G , or underwent Etv5 knockdown using siRNA H , or shRNA I . Data in this result were all representative of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, Student’s t test.

Article Snippet: Supernatants were collected after macrophage culture and IL-6 protein concentrations were measured using a mouse IL-6 ELISA kit (E-MSEL-M0001, Elabscience, China) according to the manufactories’ instructions.

Techniques: Transfection, Negative Control, Expressing, Quantitative RT-PCR, Knockdown, shRNA, Over Expression, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Bioorganic & medicinal chemistry

Article Title: Inhibition of LPS-induced inflammatory response in RAW264.7 cells by natural Chlorogenic acid isomers involved with AKR1B1 inhibition.

doi: 10.1016/j.bmc.2024.117942

Figure Lengend Snippet: Fig. 6. DARTS-based determination of affinity between CGA isomers and AKR1B1. Graph representing the change in Anti-hydrolytic stability of intracellular AKR1B1 after treatment with (a) 3-CQA, (b) 4-CQA, and (c) 5-CQA for 15 min. The concentration of AKR1B1 was determined using an ELISA kit. Data are represented as mean ± SD, n = 3. Statistically significant differences between treated cells and control group were determined by the student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: TNF-α and IL-6 ELISA kit was purchased from Elabscience (Elabscience Biotechnology Co.,Ltd.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: Bioorganic & medicinal chemistry

Article Title: Inhibition of LPS-induced inflammatory response in RAW264.7 cells by natural Chlorogenic acid isomers involved with AKR1B1 inhibition.

doi: 10.1016/j.bmc.2024.117942

Figure Lengend Snippet: Fig. 10. CETSA-based determination of target engagement between CGA isomers and AKR1B1. Graph representing the change in thermal stability of intracellular AKR1B1 after treatment with (a) 3-CQA, (b) 4-CQA, and (c) 5-CQA for 2 h. The concentration of AKR1B1 was determined using an ELISA kit. Data are represented as mean ± SD, n = 3. Statistically significant differences between treated cells and non-treated cells were determined by the student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: TNF-α and IL-6 ELISA kit was purchased from Elabscience (Elabscience Biotechnology Co.,Ltd.

Techniques: Drug discovery, Concentration Assay, Enzyme-linked Immunosorbent Assay